Stimulation of transcription on chromatin by polar organic compounds.
- 1 May 1976
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 3 (5), 1203-1214
- https://doi.org/10.1093/nar/3.5.1203
Abstract
Polar organic compounds, including DMSO, increase RNA synthesis on isolated chromatin by E. coli RNA polymerase and RNA polymerase II from calf thymus. Transcription is stimulated on chromatin from Friend-virus-infected erythroleukemia cells and from various other sources. Using procedures which inhibit specifically the formation of a stable initiation complex, it is shown that the stimulation does not result from an increase in initiation of both E. coli and the eukaryotic RNA polymerase. After separation of chromatin into template active and inactive fractions, DMSO increases RNA synthesis by a factor of about 1.5 using the template inactive fraction, while stimulation of transcription on the template active portion is lower (factor of 1.2). It is suggested that the effect on RNA synthesis is mediated by a weakening of the apolar interactions between histones in chromatin subunits, releasing transcription partially from the constraints imposed by histones.Keywords
This publication has 20 references indexed in Scilit:
- Effects of estrogen on gene expression in the chick oviduct. IV. Initiation of RNA synthesis on DNA and chromatin.Journal of Biological Chemistry, 1975
- On the Nature of DNA Promoter ConformationsEuropean Journal of Biochemistry, 1974
- Activation of Transcription at Specific Promoters by GlycerolJournal of Biological Chemistry, 1974
- Spheroid Chromatin Units (ν Bodies)Science, 1974
- Antigenic homology of eukaryotic RNA polymerasesBiochemical and Biophysical Research Communications, 1973
- Globin Messenger-RNA Induction During Erythroid Differentiation of Cultured Leukemia CellsProceedings of the National Academy of Sciences, 1972
- Synthesis of Mouse Haemoglobin and Globin mRNA in Leukaemic Cell CulturesNature New Biology, 1972
- Simple Computer-Aided Approach for the Analyses of the Nuclear-Magnetic-Resonance Spectra of Histones. Fractions Fl, F2al, F2B, Cleaved Halves of F2B and F2B middotDNAEuropean Journal of Biochemistry, 1972
- Mechanism of ribonucleic acid synthesis. II. Stabilization of the deoxyribonucleic acid-ribonucleic acid polymerase complex by the formation of a single phosphodiester bondBiochemistry, 1970
- Differential rate in RNA synthesis between euchromatin and heterochromatinExperimental Cell Research, 1962