Computer Aided Analysis of Cell-Cycle Phase from Cytophotometric Histogram

Abstract
A method for estimating, from cytophotometric data, the proportion of cells in three different phases of the cell cycle is proposed. Computation is done by means of determining seven parameters in the approximate function for the fluorescence intensity histogram. The proportion of cells in the GI, S, and G2-M phases, obtained by this method, was found to be reliable.