A photo‐CIDNP study of the active sites of Megasphaera elsdenii and Clostridium MP flavodoxins
- 1 November 1982
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 149 (1), 141-146
- https://doi.org/10.1016/0014-5793(82)81090-9
Abstract
Megasphaera elsdenii and Clostridium MP flavodoxins have been investigated by photo-CIDNP techniques. Using time-resolved spectroscopy and external dyes carrying different charges it was possible to assign unambiguously the resonance lines in the NMR-spectra to tyrosine, tryptophan and methionine residues in the two proteins. The results show that Trp-91 in M.elsdenii and Trp-90 in Cl.MP flavodoxin are strongly immobilized and placed directly above the benzene subnucleus of the prosthetic group. The data further indicate that the active sites of the two flavodoxins are extremely similar.Keywords
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