THE GAS–LIQUID PARTITION CHROMATOGRAPHY OF CARBOHYDRATE DERIVATIVES: PART III. THE SEPARATION OF AMINO GLYCOSE DERIVATIVES AND OF CARBOHYDRATE ACETAL AND KETAL DERIVATIVES

Abstract

Gas–liquid partition chromatography has been used to separate the anomeric acetates of 2-amino-2-deoxy-D-glucopyranose (D-glucosamine) and of 2-amino-2-deoxy-D-galactopyranose (D-galactosamine) and also their fully acetylated reduction products, 2-acetamido-2-deoxy-1,3,4,5,6-penta-O-acetyl-D-glucitol and 2-acetamido-2-deoxy-1,3,4,5,6-penta-O-acetyl-D-galactitol. The gas–liquid partition chromatographic method has also been successfully applied to the separation of unsubstituted carbohydrate acetal and ketal derivatives and to those derivatives in which the free hydroxyl groups have been substituted by O-acetyl, O-benzoyl, O-benzyl, carbonate, O-methanesulphonyl, O-methyl, and O-toluene-p-sulphonyl groups.