Sex pheromone biosynthesis in the red-banded leafroller moth, studied by mass-labeling with stable isotopes and analysis with mass spectrometry
- 1 February 1986
- journal article
- Published by Springer Nature in Journal of Chemical Ecology
- Vol. 12 (2), 431-450
- https://doi.org/10.1007/bf01020565
Abstract
A technique for mass-labeling was developed to study sex pheromone biosynthesis in the red-banded leafroller moth, Argyrotaenia velutinana . With this technique, the pheromone components and all fatty acyl groups in the pheromone gland were analyzed for incorporation of label in the same analytic ran with gas chromatography-mass spectrometry, using chemical ionization and selected ion monitoring (GC-SIM-CI-MS). Sex pheromone glands were incubated with fatty acids or triacylglycerols labeled with at least three deuterium atoms or carbon-13 atoms. The results of these incubations support an interpretation in which hexadecanoate is chain shortened to tetradecanoate, which is desaturated to produce ( E )- and ( Z )-11-tetradecenoate precursors for the sex pheromone components ( E )- and ( Z )-11-tetradecen-1-yl acetate. Labeled ( E )- and ( Z )-11-tetradecenoyl groups in synthetic triacylglycerols were not incorporated into the sex pheromone components, perhaps indicating that this lipid class is not a donor of the immediate fatty acyl precursors in sex pheromone biosynthesis.Keywords
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