Differential radiosensitivity of neurons and neuroglia of the hippocampus in the adult rabbit

Abstract

Adult rabbits were subjected to 4.5 Gy of whole-body or brain alone γ-irradiation, and their hippocampus was examined with the light and electron microscope. Pycnotic cells were found at the base of the granular layer of the dentate gyrus in the so-called subgranular zone, as soon as 3 h after irradiation, and were cleared up by active phagocytosis after 48 h. Some of these cells appeared as undifferentiated, whereas others were differentiating granule cells, and possibly immature neuroglia. The extent of cell necrosis was contingent upon the age of the animal, the oldest animal studied (27 months) showing only sparse lesion of that type. Astrocytes and microglia were responsible for the phagocytosis of dead cells. Another type of lesion was found in the nuclei of the mature granule cells and consisted of light spots which appeared 1 h after the irradiation and disappeared almost completely after 48 h. Pyramidal cells did not show any of these two lesions. It is concluded that the alterations in the electrical activity of pyrimidal cells, following irradiation, are at least partly due to lesions affecting the dentate gyrus. Radionecrosis in the subgranular zone is related to the presence of immature cells in this region.