A simple reliable procedure for obtaining metaphases from human leukemic bone-marrow aspirates suitable for Giemsa banding
- 1 February 1980
- journal article
- research article
- Published by Springer Nature in In Vitro Cellular & Developmental Biology - Plant
- Vol. 16 (2), 109-112
- https://doi.org/10.1007/bf02831501
Abstract
Short incubation of heparinized human leukemic bone-marrow cells in phosphate buffered saline containing colcemid and overnight chilling of fixed cells yields metaphases with elongated and well-spread chromosomes. This technique enables us to do trypsin-Giemsa banding of chromosomes obtained from leukemic marrow cells otherwise difficult to band.This publication has 5 references indexed in Scilit:
- Bone-marrow chromosomes in healthy subjectsHereditas, 2009
- An improved technique of preparing bone-marrow specimens for cytogenetic analysisIn Vitro Cellular & Developmental Biology - Plant, 1978
- SALINE WASHES STIMULATE THE PROLIFERATION OF HUMAN FIBROBLASTS IN CULTUREPublished by Elsevier ,1977
- Identification of chromosome anomalies in the blastic phase of chronic myelocytic leukemia (CML) by Giemsa- and quinacrine-banding techniquesHuman Genetics, 1974
- A RAPID BANDING TECHNIQUE FOR HUMAN CHROMOSOMESThe Lancet, 1971