Isolation of an actin-binding fragment of fibronectin
- 1 February 1981
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 193 (2), 615-620
- https://doi.org/10.1042/bj1930615
Abstract
A specific actin-binding site in the adhesive glycoprotein fibronectin, isolated from chicken fibroblasts, was identified. Affinity chromatography of fragments, released from fibronectin by limited proteolysis with trypsin, chymotrypsin and subtilisin, on actin-Sepharose and other protein-Sepharose columns was used to locate the binding site. A 27,000 MW subtilisin-digest fragment bound efficiently to actin. The results suggest that the actin-binding site is close to, but not identical with, the reported collagen-binding site.This publication has 38 references indexed in Scilit:
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