Changes in Guinea‐Pig Dermal Collagen during Development
Open Access
- 1 July 1975
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 55 (2), 391-395
- https://doi.org/10.1111/j.1432-1033.1975.tb02174.x
Abstract
Guinea-pig dermis was digested with pepsin and the solubilized collagen molecules separated by differential salt precipitation at pH 7.5. Differences in subunit composition and amino acid analysis were noted between type I and type III collagen. Incorporation of radioactive proline into the developing foetus enabled isolation of labelled type I and type III collagens. Comparison of the specific activity of the isolated collagen molecules showed that type III collagen had a high specific activity in the early stages of foetal development, which decreased dramatically during foetal development. The specific activity of pepsin-solubilized type I collagen remained fairly constant during foetal development.Keywords
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