Elution patterns of rubella IgM, IgA, and IgG antibodies from a dextran and an agarose gel.
- 1 August 1975
- journal article
- Published by BMJ in Journal of Clinical Pathology
- Vol. 28 (8), 670-673
- https://doi.org/10.1136/jcp.28.8.670
Abstract
The elution pattern of serum proteins and the distribution of rubella HAI activity in 95 sera from 65 cases were determined after gel filtration with (a) Sephadex G-200 and (b) Bio-Gel A-5M. Rubella HAI antibody in peak 1 after Sephadex G-200 fractionation of early convalescent sera consists of both IgM and high molecular weight IgA. However, these two classes of antibody can be distinguished by gel filtration with Bio-Gel A-5M. Bearing these differences in mind, the results of fractionation with the two gels correlate very well although the use of Bio-Gel A-5M gives a slightly less sensitive technique for the diagnosis of recent infection.Keywords
This publication has 7 references indexed in Scilit:
- The detection of specific IgM antibodies following infection with rubella virus.Journal of Clinical Pathology, 1975
- PERSISTENCE OF SPECIFIC IgM AFTER NATURAL INFECTION WITH RUBELLA VIRUSThe Lancet, 1975
- A simplified method for the detection of rubella-specific IgM employing sucrose density fractionation and 2-mercaptoethanolEpidemiology and Infection, 1974
- A simple, inexpensive gel filtration technique for use in diagnostic serology.Journal of Clinical Pathology, 1973
- SEPARATION OF RUBELLA IgM, IgA, AND IgG ANTIBODIES BY GEL FILTRATION ON AGAROSEThe Lancet, 1971
- Single-sample diagnosis of recent rubella by fractionation of antibody on Sephadex G-200 columnJournal of Clinical Pathology, 1971
- SERUM IgM AND IgG RESPONSES IN POSTNATALLY ACQUIRED RUBELLAThe Lancet, 1969