Separation of Fluorescent Antibody Conjugates into 7S and 19S Globulin Components by Gel Filtration.

Abstract

Summary Gel filtration proved to be a simple, effective method for separation of fluorescein-labeled antiglobulin for E. coli into 7S and 19S fractions. Both fractions were found to contribute considerable nonspecific staining, while specific staining was associated predominantly with the 7S globulin. The ratio of specific staining titer to nonspecific staining activity was 3-fold greater with a pool of fractions in which only 7S globulin was detected than with the unfractionated conjugate when the 2 preparations were tested at the same protein concentration.