Hormonal control of muscle growth

Abstract

In muscle of whole animals, pituitary growth hormone, the thyroid hormones, and insulin are major growth‐promoting hormones, and the glucocorticoids have significant catabolic actions. At the cellular level the primary anabolic hormones for cultured myoblasts are the somatomedins (insulin‐like growth factors) and fibroblast growth factor. In these cells physiological concentrations of growth hormone, thyroid hormones, and insulin have no growth‐promoting effect; some of the reported actions of insulin probably result from cross‐reaction with the somatomedin receptor. Results with purified proteins do not support the view that mitogens block myoblast differentiation; transforming growth factor‐beta and interferon are nonmitogenic proteins that inhibit differentiation, insulin‐like growth factors are mitogens that stimulate differentiation, and fibroblast growth factor is the only purified mitogen that inhibits differentiation. At least six serum‐free media have now been devised for the growth of various kinds of muscle cells under closely defined conditions.