Cloning of Wheat LTP1500 and TwoFusarium culmorumHydrophobins inSaccharomyces cerevisiaeand Assessment of Their Gushing Inducing Potential in Experimental Wort Fermentation
Open Access
- 1 January 2006
- journal article
- research article
- Published by The Institute of Brewing & Distilling in Journal of the Institute of Brewing
- Vol. 112 (3), 237-245
- https://doi.org/10.1002/j.2050-0416.2006.tb00719.x
Abstract
The potential of surface active proteins to affect gushing upon their formation in situ during fermentation was investigated. This was achieved by cloning the genes of two hydrophobins of F. culmorum and of a wheat lipid transfer protein (LTP1500) in Saccharomyces cerevisiae, with expression of these genes under control of the constitutive TPI (triose phosphate isomerase) promoter. The transgenic yeast clones were used for fermentation of wort. The resulting beers were bottled and examined for the occurrence of gushing. Gushing was induced by the class II hydrophobin FcHyd5p of the fungus F. culmorum, found in naturally occurring cases of gushing worldwide.Keywords
This publication has 11 references indexed in Scilit:
- TheTrichoderma reeseihydrophobin geneshfb1andhfb2have diverse functions in fungal developmentFEMS Microbiology Letters, 2005
- Common resistance to different Fusarium spp. causing Fusarium head blight in wheatEuropean Journal of Plant Pathology, 2005
- Fungal Hydrophobins as Predictors of the Gushing Activity of MaltJournal of the Institute of Brewing, 2005
- Five hydrophobin genes in Fusarium verticillioides include two required for microconidial chain formationFungal Genetics and Biology, 2004
- Are Hydrophobins and/or Non-Specific Lipid Transfer Proteins Responsible for Gushing in Beer? New Hypotheses on the Chemical Nature of Gushing Inducing FactorsZeitschrift für Naturforschung C, 2002
- Purification and Structural Characterization of LTP1 Polypeptides from BeerJournal of Agricultural and Food Chemistry, 2000
- Fusarium head blight pathogens isolated from fusarium-damaged kernels of wheat in western Canada, 1993 to 1998Canadian Journal of Plant Pathology, 2000
- Applications of high efficiency lithium acetate transformation of intact yeast cells using single‐stranded nucleic acids as carrierYeast, 1991
- High efficiency transformation of Escherichia coli with plasmidsGene, 1990
- Rapid preparation of DNA from filamentous fungiLetters in Applied Microbiology, 1985