Chromatography on ECTEOLA of Sulfate Containing Mucopolysaccharides.
- 1 January 1960
- journal article
- research article
- Published by Danish Chemical Society in Acta Chemica Scandinavica
- Vol. 14 (2), 303-311
- https://doi.org/10.3891/acta.chem.scand.14-0303
Abstract
The chromatographic behavior at different pH of hyaluronic acid, chrondroitinsulfuric acid and heparin on ECTEOLA cellulose columns was studied. At acid pH good separations of the three polysaccharides from each other were obtained either by step-wise or gradient elution chromatography. Heparin preparations could be fractionated into sub-fractions of different anticoagulant activity. When the chromatography was carried out at +4[degree]C no detectable inactivation of heparin occurred. The recovery of polysaccharides from the columns was close to 100%.This publication has 7 references indexed in Scilit:
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