Comparison of G-, Q-, and EM-banding patterns exhibited by the chromosome complement of the Indian muntjac, Muntiacus muntjak, with reference to nuclear DNA content and chromatin ultrastructure

Abstract

When the chromosomes of the Indian muntjac, Muntiacus muntjak, were compared following treatment with two presently used banding methods, trypsin-Giemsa (G) and quinacrine-hydrochloride (Q) with structural bands as seen in the electron microscope, definite correlations were observed with respect to the numbers and positions of individual bands. — Weights obtained for the individual chromosomes were: No. 1, 9.89 pg; No. 2, 4.10 pg; No. 3, 4.43 pg; No. 3−X, 5.05 pg; and Y, 0.55 pg. Average diameters and weights for individual fibers were 193 Å, and 8.74×10⁻¹⁶g/μ, respectively, for stimulated metaphase chromosomes and 185 Å and 8.73 × 10⁻¹⁶ g/μ, respectively, for unstimulated chromosomes. Fibers of interphase nuclei exhibited an average diameter of 191 Å and a weight of 5.87×10⁻¹⁶ g/μ. — The total amount of nuclear DNA present in interphase nuclei was 3.88 pg.