Aspects on diamine oxidase activity and its determination
- 1 February 1978
- journal article
- research article
- Published by Wiley in Acta Physiologica Scandinavica
- Vol. 102 (2), 159-166
- https://doi.org/10.1111/j.1748-1716.1978.tb06059.x
Abstract
Homogenate of guinea pig liver and human placenta, tissues known to be rich in diamine oxidase [EC 1.4.3.6], were incubated with 14C-putrescine and the metabolites formed were determined. In the incubate of guinea pig liver, the major metabolites were GABA and some unidentified compound(s); .DELTA.1-pyrroline and 14CO2 were also obtained. In both the maternal and fetal parts of human placenta, radioactive GABA and the unidentified compound(s) as well as .DELTA.1-pyrroline were found. GABA is an important intermediate in putrescine metabolism. Determination of the amount of .DELTA.1-pyrroline is thus not well suited as a measure of the diamine oxidase activity in tissues.Keywords
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