An Artificial Reagent for the Diagnosis of Classical Hemophilia

Abstract

Normal plasma, collected in disodium ethylenediaminetetraacetic acid, is aged for 30 days at 4[degree]C. After the addition of Asolectin and barium sulfate adsorbed oxalated bovine serum, it is used as a testing reagent. When mixed with sera defective in antithemophilia globulin, it takes a longer time to clot (6 1/2-15 minutes) than with normal serum or sera deficient in other components (2 1/2-5 minutes).