Effects of Insulin on the Permeability of D- and L-Xylose and D- and L-Arabinose in Rat Diaphragm Muscle
Open Access
- 1 November 1961
- journal article
- research article
- Published by Rockefeller University Press in The Journal of general physiology
- Vol. 45 (2), 309-316
- https://doi.org/10.1085/jgp.45.2.309
Abstract
The permeability characteristics of D- and L-xylose and D- and L-arabinose have been compared in isolated intact rat diaphragm muscle preparations, in the absence and presence of exogenous insulin. In the absence of added insulin, these pentoses distribute in less than a third of the total cell water. In the presence of added insulin, intracellular distribution of all these pentoses is increased. L-Xylose and D-arabinose distribute in 50 per cent of the intracellular water, whereas D-xylose and L-arabinose distribute in 80 per cent of the cell water. A significant lag period was observed before the insulin effect upon the penetration of L-xylose and D-arabinose was evident whereas the effect upon D-xylose and L-arabinose was rapid. The lag period with L-xylose could be abolished by pretreating the tissues with insulin for 1 hour, but such pretreatment had little effect on D-xylose. These results indicate that insulin has a biphasic effect upon the monosaccharide exclusion system in diaphragm muscle. In dinitrophenol-treated tissues, in which all permeability processes are irreversibly damaged and in which sucrose and pentoses penetrate into most of the cell water, the entry rate of pentoses and sucrose is initially similar but subsequently D-xylose and L-arabinose penetrate more rapidly than their corresponding optical isomers.Keywords
This publication has 11 references indexed in Scilit:
- Regulation of glucose uptake by muscle. 4. The specificity of monosaccharide-transport systems in rat-diaphragm muscleBiochemical Journal, 1960
- STUDIES OF TISSUE PERMEABILITY .7. EFFECT OF INSULIN ON GLUCOSE PENETRATION AND PHOSPHORYLATION IN FROG MUSCLE1960
- Osmotic effects and diffusion of nonelectrolytes in muscleAmerican Journal of Physiology-Legacy Content, 1959
- ACTION OF INSULIN ON SUGAR PERMEABILITY IN RAT DIAPHRAGM MUSCLEThe Journal of general physiology, 1959
- Regulation of glucose uptake by muscle. 2. The effects of insulin, anaerobiosis and cell poisons on the penetration of isolated rat diaphragm by sugarsBiochemical Journal, 1958
- Conformational Specificity in a Biological Sugar Transport SystemAmerican Journal of Physiology-Legacy Content, 1958
- Insulin and Tissue Distribution of Pentose in Nephrectomized CatsAmerican Journal of Physiology-Legacy Content, 1957
- STUDIES OF TISSUE PERMEABILITY .2. THE DISTRIBUTION OF PENTOSES BETWEEN PLASMA AND MUSCLE1957
- STUDIES OF TISSUE PERMEABILITY .3. THE EFFECT OF INSULIN ON PENTOSE UPTAKE BY THE DIAPHRAGM1957
- Speculations on the Action of Insulin, With a Note on Other Hypoglycemic AgentsPerspectives in Biology and Medicine, 1957