Identification of an Annexin‐Like Protein and Its Possible Role in the Aplysia Eye Circadian System
- 1 October 1993
- journal article
- Published by Wiley in Journal of Neurochemistry
- Vol. 61 (4), 1236-1245
- https://doi.org/10.1111/j.1471-4159.1993.tb13614.x
Abstract
Light and serotonin regulate the phase of the circadian rhythm of the isolated eye of Aplysia. To screen for possible protein components of the eye circadian oscillator, we identified a number of proteins whose synthesis was altered in opposite ways by light and serotonin. The cellular function of one of these proteins was investigated by obtaining a partial amino acid sequence of it and by examining its immunoreactivity. A 38-amino acid sequence was obtained from a 40-kDa (isoelectric point 5.6) protein. A greater than 60% amino acid identity existed between this sequence and sequences of a family of calcium/phospholipid-binding proteins called annexins. Furthermore, the 40-kDa protein reacted with antibodies generated against a conserved amino acid sequence of annexins and with antibodies raised against human annexin I. The identification of the 40-kDa, light- and serotonin-regulated protein as an annexin led us to hypothesize that arachidonic acid metabolism plays a role in the Aplysia eye circadian system. To test this hypothesis, we examined the ability of an inhibitor of the arachidonic acid metabolic pathway to perturb the eye rhythm. Pulse treatments of isolated eyes with a lipoxygenase inhibitor, nordihydroguaiaretic acid, phase shifted the rhythm. The phase-shifting ability of nordihydroguaiaretic acid suggests that arachidonic acid and some of its metabolites may play a role in the eye circadian system. The results of our studies raise the possibility that links may exist between the 40-kDa annexin-like protein, arachidonic acid metabolism, and the circadian oscillator.Keywords
This publication has 64 references indexed in Scilit:
- The Hunt for Mechanisms of Circadian Timing in the Eye of AplysiaChronobiology International, 1992
- Synexin (annexin VII): A cytosolic calcium-binding protein which promotes membrane fusion and forms calcium channels in artificial bilayer and natural membranesThe Journal of Membrane Biology, 1990
- Cycloheximide Inhibits Light-Induced Phase Shifting of the Circadian Clock in NeurosporaJournal of Biological Rhythms, 1990
- Affinity-purified site-directed antibody recognizes the entire annexin protein familyBiochemical and Biophysical Research Communications, 1989
- The annexin family of calcium-binding proteins: Review articleCell Calcium, 1989
- Calpactins: two distinct Ca++-regulated phospholipid- and actin-binding proteins isolated from lung and placenta.The Journal of cell biology, 1987
- Common domain structure of Ca2+ and lipid‐binding proteinsFEBS Letters, 1986
- Activation of soluble guanylate cyclase by arachidonic acid and 15-lipoxygenase productsBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1986
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Circadian Rhythm of Optic Nerve Impulses Recorded in Darkness from Isolated Eye of AplysiaScience, 1969