Titrimetric Analysis of Calcium and Magnesium in Muscle.

Abstract

Relatively simple EDTA titration methods for the determination of muscle calcium and magnesium are given. Calcium is precipitated with ammonium oxalate; the oxalate is destroyed by heating with perchloric acid; and then the calcium is titrated with EDTA using either calcein or Calred as the indicator. Magnesium is precipitated with oxine; the oxine is destroyed by heating with perchloric acid; then sufficient EDTA is added in the presence of either calcein or Calred until the color of the indicator changes, after which the magnesium is titrated with additional EDTA using Eriochrome Black T as the indicator.