Microscale method for rapid isolation of covalently closed circular plasmid DNA from group N streptococci
- 1 April 1984
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 47 (4), 677-680
- https://doi.org/10.1128/aem.47.4.677-680.1984
Abstract
A method for rapid purification of plasmid DNA from lactic streptococci, utilizing microliter quantities of reagents, was developed by combination of a short lysozyme-mutanolysin cell wall digestion with a modification of the Escherichia coli plasmid isolation procedure of McMaster et al. (Anal. Biochem. 109:47-54, 1980). The preparations obtained were highly enriched for covalently closed circular DNA, and the method was applicable to plasmids of at least 40 megadaltons. Centrifugation in CsCl-ethidium bromide density gradients was not required.This publication has 28 references indexed in Scilit:
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