The characterization and purification of DNA binding proteins present within herpes simplex virus infected cells using monoclonal antibodies
- 1 March 1984
- journal article
- Published by Springer Nature in Archiv für die gesamte Virusforschung
- Vol. 79 (1-2), 13-33
- https://doi.org/10.1007/bf01314300
Abstract
Hybridomas secreting monoclonal antibodies against herpes simplex virus (HSV) DNA binding proteins (DBP) have been produced. Five HSV DBP have been characterized according to molecular weight, affinity for DNA, kinetic class and localization within the infected cell. By preparing an immunoadsorbent column from antibody TI8, its specific DBP was purified to apparent homogeneity. The purified DBP retained the ability to bind to DNA.Keywords
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