Modification of the Acid Elution Technique for Quantitation of Fetal Hemoglobin in Individual Erythrocytes
- 1 January 1977
- journal article
- research article
- Published by Taylor & Francis in Hemoglobin
- Vol. 1 (4), 313-331
- https://doi.org/10.3109/03630267708996893
Abstract
A modification of the acid elution procedure for the demonstration of fetal Hb in red cells using fast green stain is described. This technique not only permits improved visual estimation of the amount of fetal Hb in red cells, but allows for the direct quantitation of fetal Hb content of individual cells, using a scanning and integrating microdensitometer. As an application of this method, the distribution of fetal Hb in populations of red cells of individuals was determined. Distributions were examined in sickle cell anemia patients with different proportions of fetal Hb.This publication has 24 references indexed in Scilit:
- A new form of hereditary persistence of fetal hemoglobin in blacks and its association with sickle cell traitBlood, 1975
- Quantitation of hemoglobins by immunodiffusion: Specific antibodies to hemoglobins A1, S and FImmunochemistry, 1974
- Immunological quantification of hemoglobins F and A2Clinica Chimica Acta; International Journal of Clinical Chemistry, 1974
- An immunological method for the detection and estimation of fetal haemoglobin.Journal of Clinical Pathology, 1972
- Studies on hemoglobin F within single erythrocyte by fluorescent antibody techniqueExperimental Cell Research, 1965
- Demonstration of Fœtal Erythrocyte by Immunofluorescent StainingNature, 1964
- Estimation of Small Percentages of Fœtal HæmoglobinNature, 1959
- Serological Distinction of Adult and Fœtal Human HæmoglobinNature, 1959
- Demonstration von fetalem H moglobin in den Erythrocyten eines BlutausstrichsKlinische Wochenschrift, 1957
- Studies on Abnormal HemoglobinsBlood, 1951