EFFECT OF TRYPSIN INHIBITORS AND CONCANAVALIN A ON BUFO ARENARUM FERTILIZATION

Abstract

The presence of some trypsin inhibitors (soybean trypsin inhibitor and lima bean trypsin inhibitor) in the inseminating media, is effective in blocking B. arenarum fertilization. Ovomucoid does not significantly affect fertilization, even at concentrations as high as 10 mg/ml. Similarly, the presence of concanavalin A in the inseminating media results in fertility impairment. The possible mechanisms of action of these substances are discussed.