Movement of Retinal Along the Visual Transduction Path
- 23 June 2000
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 288 (5474), 2209-2212
- https://doi.org/10.1126/science.288.5474.2209
Abstract
Movement of the ligand/receptor complex in rhodopsin (Rh) has been traced. Bleaching of diazoketo rhodopsin (DK-Rh) containing 11- cis -3-diazo-4-oxo-retinal yields batho-, lumi-, meta-I-, and meta-II-Rh intermediates corresponding to those of native Rh but at lower temperatures. Photoaffinity labeling of DK-Rh and these bleaching intermediates shows that the ionone ring cross-links to tryptophan-265 on helix F in DK-Rh and batho-Rh, and to alanine-169 on helix D in lumi-, meta-I-, and meta-II-Rh intermediates. It is likely that these movements involving a flip-over of the chromophoric ring trigger changes in cytoplasmic membrane loops resulting in heterotrimeric guanine nucleotide–binding protein (G protein) activation.Keywords
This publication has 34 references indexed in Scilit:
- Combined biophysical and biochemical information confirms arrangement of transmembrane helices visible from the three-dimensional map of frog rhodopsinJournal of Molecular Biology, 1998
- An alpha-carbon template for the transmembrane helices in the rhodopsin family of G-protein-coupled receptors 1 1Edited by R. HuberJournal of Molecular Biology, 1997
- The steric trigger in rhodopsin activationJournal of Molecular Biology, 1997
- Rhodopsin activation blocked by metal-ion-binding sites linking transmembrane helices C and FNature, 1996
- Specific Tryptophan UV-Absorbance Changes Are Probes of the Transition of Rhodopsin to Its Active StateBiochemistry, 1996
- Molecular determinants of human red/green color discriminationNeuron, 1994
- Circular Dichroism of Metaiodopsin II and Its Binding to Transducin: A Comparative Study between Meta II Intermediates of Iodopsin and RhodopsinBiochemistry, 1994
- Nanosecond photolysis of rhodopsin: evidence for a new blue-shifted intermediateBiochemistry, 1990
- Complete assignment of the hydrogen out-of-plane wagging vibrations of bathorhodopsin: chromophore structure and energy storage in the primary photoproduct of visionBiochemistry, 1989
- Fourier-transform infrared difference spectroscopy of rhodopsin and its photoproducts at low temperatureBiochemistry, 1985