EFFECT OF ADRIAMYCIN AND ANALOGS ON NUCLEAR FLUORESCENCE OF PROPIDIUM IODIDE STAINED CELLS

Abstract

Adriamycin (ADR) and N-trifluoroacetyladriamycin-14-valerate, respectively, inhibit and enhance the nuclear fluorescence of [human CCRF-CEM lymphoblasts and mouse P 388 leukemia] cells stained with propidium iodide for DNA per cell estimation by flow cytometry. In cells incubated with ADR, the reduction in fluorescence is gradually manifested due to the slow intracellular drug transport. In contrast the effect of N-trifluoroacetyladriamycin-14-valerate on propidium iodide nuclear fluorescence is seen within 5 min of incubation. The effect of ADR on propidium iodide nuclear fluorescence could be detected in vivo even after 24 h of ADR administration.