Nonrandom Ectopic Protein Production by Malignant Cells: Direct Evidence in Vitro

Abstract

A large number of cell lines derived from malignant human neoplasms (21 carcinomas or melanomas and 11 sarcomas or gliomas) and 18 human fibroblast lines were examined for ectopic protein production by RIA. By conservative criteria, 13 malignant lines produced ectopic CG or its ß-subunit (0.5–5.6 pmol/mg cell protein). Four of these lines produced ectopiccarcinoembryonic antigen (0.04–0.95), while none produced placental lactogen or ∞-fetoprotein (progesterone, 17ßhydroxyprogesterone, testosterone, or aldosterone. Estrone andestradiol were found in media from 4 malignant lines (1.1–9.4 pmol/mg protein). These data suggest that ectopic protein production is 1) widely prevalent in malignant cells in culture and rare in normal fibroblast cultures, 2) nonrandom, with large amounts of certain proteins butundetectable amounts of others, and 3) underestimated by criteria of serum concentration alone, since certain lines produce low levels or do not secrete. The multiple enzymes required for stefoidogenesis are not produced together ectopically, but estrogen production by cells in media containing serum may require only a singlearomatase or desul-furylase. (J. Clin Endocrinol Metab50: 834, 1980)