Interferon gamma and interleukin 4 secreting cells in the gastric antrum in Helicobacter pylori positive and negative gastritis.
Open Access
- 1 March 1995
- Vol. 36 (3), 341-345
- https://doi.org/10.1136/gut.36.3.341
Abstract
Little is known of the function of the T cells in the inflammatory infiltrate in Helicobacter pylori associated gastritis. This study thus measured T cell in vivo activation by enumerating the frequency of interferon gamma (IFN gamma) and interleukin 4 (IL 4) secreting cells isolated from the gastric antral mucosa in patients with or without gastritis and in H pylori positive and negative gastritis. Fifty four samples were examined for cytokine secretion. Four antral biopsy specimens from each patient (n = 51) were taken during diagnostic endoscopy. One was used to estimate histological gastritis and the presence of H pylori, and three of the samples were used to isolate T cells by enzymatic digestion. IFN gamma and IL 4 secreting cells were enumerated by ELISPOT. Thirty four samples had gastritis and 79% of those were H pylori positive. None of the samples from non-inflamed mucosa had H pylori. The numbers of IFN gamma secreting cells per 10(5) T cells were higher in gastritis than in normal mucosa (145 v 20 IFN gamma spots, p < 0.01), and higher in H pylori negative than H pylori positive gastritis (371 v 110 IFN gamma spots, p < 0.05). The frequencies of IL 4 secreting cells did not differ between gastritis and non-inflamed mucosa. In conclusion, there is an increase in IFN gamma secreting cells but not in IL 4 secreting cells in H pylori positive and negative gastritis. It is not known if this TH1 type reaction has a pathogenetic or protective role.Keywords
This publication has 19 references indexed in Scilit:
- Role of T‐Cell Derived Cytokines in the Downregulation of Immune Responses in Parasitic and Retroviral InfectionImmunological Reviews, 1992
- Mucosal tumour necrosis factor alpha and interleukin-6 in patients with Helicobacter pylori associated gastritis.Gut, 1991
- Defining Protective Responses to Pathogens: Cytokine Profiles in Leprosy LesionsScience, 1991
- Intestinal immune responses in humans. Oral cholera vaccination induces strong intestinal antibody responses and interferon-gamma production and evokes local immunological memory.JCI Insight, 1991
- Blood lymphocyte proliferation, cytokine secretion and appearance of T cells with activation surface markers in cultures with Helicobacter pylori. Comparison of the responses of subjects with and without antibodies to H. pyloriClinical and Experimental Immunology, 1991
- Helicobacter pyloriin Dyspeptic Patients: Quantitative Association with Severity of Gastritis, Intragastric pH, and Serum Gastrin ConcentrationScandinavian Journal of Gastroenterology, 1991
- Helicobacter pylori induces lymphocyte activation in peripheral blood culturesClinical and Experimental Immunology, 1990
- Relation between T cell number and epithelial HLA class II expression quantified by image analysis in normal and inflamed human gastric mucosa.Gut, 1990
- Detection of intracellular expression and secretion of interferon‐γ at the single‐cell level after activation of human T cells with tetanus toxoid in vitroEuropean Journal of Immunology, 1990
- Interferon-gamma directly affects barrier function of cultured intestinal epithelial monolayers.JCI Insight, 1989