The biosynthesis of rubber: Incorporation of isopentenyl pyrophosphate into purified rubber particles by a soluble latex-serum enzyme
- 1 October 1966
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 101 (1), 42-47
- https://doi.org/10.1042/bj1010042
Abstract
1. The rubber particles in Hevea brasiliensis latex have been partially purified by; washing' with buffer solution, and separated into active fractions of different particle size. 2. The enzyme responsible for incorporating isopentenyl pyrophosphate into rubber is distributed between the surface of the rubber particles and the aqueous serum phase of the latex. The enzyme at the surface can be removed or inactivated if the rubber particles are washed sufficiently with buffer solution. Enzyme in the serum phase can be concentrated by fractional precipitation with ammonium sulphate. 3. To incorporate isopentenyl pyrophosphate into rubber in vitro, active rubber particles are required as well as enzyme and soluble cofactors. The activity of the rubber particles per unit surface area increases with diminishing particle size.This publication has 5 references indexed in Scilit:
- The biosynthesis of rubber. Incorporation of mevalonate and isopentenyl pyrophosphate into rubber by Hevea brasiliensis-latex fractionsBiochemical Journal, 1963
- Particulate ribonucleoprotein components of Hevea brasiliensis latexBiochemical Journal, 1962
- Chemical Oxidation of Uridine Diphosphate-Glucose to Uridine Diphosphate-Glucuronic AcidNature, 1961
- BIOSYNTHESIS OF TERPENES .7. ISOPENTENYL PYROPHOSPHATE ISOMERASE1960
- Nucleotides of Hevea brasiliensis Latex. A ribonucleoprotein componentBiochemical Journal, 1959