Construction of a Shuttle Vector for Inducible Gene Expression in Escherichia coli and Bacillus subtilis
- 1 March 1988
- journal article
- research article
- Published by Microbiology Society in Microbiology
- Vol. 134 (3), 605-609
- https://doi.org/10.1099/00221287-134-3-605
Abstract
The construction of a shuttle vector for inducible gene expression allowing fast and easy cloning in Escherichia coli and subsequent transformation of Bacillus subtilis is presented. The expression is based on the regulation of the tac promoter by the Lac repressor which was assayed with the xylE gene from Pseudomonas putida as a marker gene. The lacIq gene, transcribed by the strong spo promoter, allowed full repression of the weak tac promoter.This publication has 16 references indexed in Scilit:
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