Ultrastructural evidence for myosin of the smooth muscle type at the surface of trypsin-dissociated embryonic chick cells
Open Access
- 1 July 1974
- journal article
- Published by The Company of Biologists in Journal of Cell Science
- Vol. 15 (2), 279-289
- https://doi.org/10.1242/jcs.15.2.279
Abstract
Cells dissociated from embryonic chick muscle tissue using trypsin were rotated in the presence of globulin-enriched rabbit antisera against both sm∞th and striated muscle actomyosins originating from chicken gizzard (GAM) and pectoralis (PAM) muscles respectively. The presence of the rabbit antibodies was demonstrated using peroxidase-labelled sheep antirabbit γ-globulins, the enzyme-antibody conjugate being located by electron-microscope histochemistry. Anti-GAM γ-globulins reacted strongly with the plasma membrane. Judging from the complete absence of staining, γ-globulins from non-immunized rabbit serum did not interact with the membrane. When γ-globulins of sheep anti-rabbit IgG serum were applied alone, that is in the absence of pretreatment with rabbit γ-globulin, there was an observable reaction with the cell surface. Preincubation of anti-GAM with the heavy meromyosin fraction from smooth-muscle myosin inhibited the interaction of the antibodies with the membrane, as evidenced by the absence of staining. A weak positive reaction obtained with anti-PAM was due to components of the antibody preparation which were reactive with actin and not with PAM. It was concluded that a smooth-muscle myosin-like protein is an integral part of the plasma membrane of embryonic chick muscle cells.Keywords
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