Mutational analysis of a plant heat shock element
Open Access
- 1 July 1992
- journal article
- Published by Springer Nature in Plant Molecular Biology
- Vol. 19 (4), 665-675
- https://doi.org/10.1007/bf00026792
Abstract
A total of 32 mutations were generated within the TATA-proximal site 1 (−72 to−47) of soybean heat shock gene Gmhsp17.5E in order to functionally define the optimal configuration of sequences within the heat shock element (HSE). Mutants were tested in vivo utilizing sunflower tumors transformed by a T-DNA based vector. Promoter activity was determined by S1 nuclease hybrid protection analysis of tumor transcripts. A total of five repeats (5′-nGAAn-3′ or 5′-nTTCn-3′) which comprise the HSE at site 1 were required for full transcription induction by heat stress. Analysis of non-conserved bases flanking the central trinucleotide block indicated that 5′-aGAAg′-3′ is the optimum sequence for the 5 bp repeat.Keywords
This publication has 34 references indexed in Scilit:
- Cooperative binding of drosophila heat shock factor to arrays of a conserved 5 bp unitCell, 1991
- Transcriptional regulation of the heat-shock response: a plant perspectiveBiochemistry, 1991
- Molecular cloning and expression of a hexameric Drosophila heat shock factor subject to negative regulationCell, 1990
- In Vitro Interaction of Nuclear Proteins with the Promoter of Soybean Heat Shock Gene Gmhsp17.5EPlant Physiology, 1990
- Trimerization of a yeast transcriptional activator via a coiled-coil motifCell, 1989
- Stable binding of Drosophila heat shock factor to head-to-head and tail-to-tail repeats of a conserved 5 bp recognition unitCell, 1989
- Nucleotide sequence analysis of soybean small heat shock protein genes belonging to two different multigene familiesJournal of Molecular Biology, 1988
- Studies on transformation of Escherichia coli with plasmidsJournal of Molecular Biology, 1983
- Three regions upstream from the cap site are required for efficient and accurate transcription of the rabbit β-globin gene in mouse 3T6 cellsCell, 1983
- Screening λgt Recombinant Clones by Hybridization to Single Plaques in SituScience, 1977