Antiviral and Anticellular Activities of Human and Murine Type I and Type II Interferons in Human Cells Monosomic, Disomic, and Trisomic for Chromosome 21

Abstract

Two trisomic-21 (T-21), 1 monosomic-21 (M-21) and 3 diploid (D-21) human fibroblast cell strains were compared for their sensitivity to the antiviral and anticellular activities of human type I (HuIFN-.beta.), human type II (HuIFN-.gamma.), murine type I (MuIFN-.beta.) and murine type II (MuIFN-.gamma.) interferons. Antiviral activity was monitored by the reduction of vesicular stomatitis virus yield and anticellular activity was assessed by the inhibition of [methyl-3H]thymidine uptake in proliferating cells. T-21 cells were more sensitive to the antiviral activities of HuIFN-.beta. and MuIFN-.beta. than either D-21 or M-21 cells. T-21 cells were not more sensitive to the antiviral activities of HuIFN-.gamma. and MuIFN-.gamma.. Neither did T-21 cells exhibit an increased sensitivity to the anticellular activity of either HuIFN-.beta. or MuIFN-.beta. or -.gamma.. A chromosome 21 dosage effect could not be observed under any of the test conditions.