Differential Effects of Growth Factors on Tissue-Engineered Cartilage
- 1 February 2002
- journal article
- research article
- Published by Mary Ann Liebert Inc in Tissue Engineering
- Vol. 8 (1), 73-84
- https://doi.org/10.1089/107632702753503072
Abstract
The effects of four regulatory factors on tissue-engineered cartilage were examined with specific focus on the ability to increase construct growth rate and concentrations of glycosaminoglycans (GAG) and collagen, the major extracellular matrix (ECM) components. Bovine calf articular chondrocytes were seeded onto biodegradable polyglycolic acid (PGA) scaffolds and cultured in medium with or without supplemental insulin-like growth factor (IGF-I), interleukin-4 (IL-4), transforming growth factor-β1 (TGF-β1) or platelet-derived growth factor (PDGF). IGF-I, IL-4, and TGF-β1 increased construct wet weights by 1.5-2.9-fold over 4 weeks of culture and increased amounts of cartilaginous ECM components. IGF-I (10-300 ng/mL) maintained wet weight fractions of GAG in constructs seeded at high cell density and increased by up to fivefold GAG fractions in constructs seeded at lower cell density. TGF-β1 (30 ng/mL) increased wet weight fractions of total collagen by up to 1.4-fold while maintaining a high fraction of type II collagen (79 ± 11% of the total collagen). IL-4 (1-100 ng/mL) minimized the thickness of the GAG-depleted region at the construct surfaces. PDGF (1-100 ng/mL) decreased construct growth rate and ECM fractions. Different regulatory factors thus elicit significantly different chondrogenic responses and can be used to selectively control the growth rate and improve the composition of engineered cartilage.Keywords
This publication has 32 references indexed in Scilit:
- Mammalian Chondrocytes Expanded in the Presence of Fibroblast Growth Factor 2 Maintain the Ability to Differentiate and Regenerate Three-Dimensional Cartilaginous TissueExperimental Cell Research, 1999
- Effect of Serum and Platelet-Derived Growth Factor on Chondrocytes Grown in Collagen GelsTissue Engineering, 1999
- Coordinate upregulation of cartilage matrix synthesis in fibrin cultures supplemented with exogenous insulin‐like growth factor‐IJournal of Orthopaedic Research, 1999
- Effects of growth factors and interleukin-1 alpha on proteoglycan and type II collagen turnover in bovine nasal and articular chondrocyte pellet culturesEndocrinology, 1996
- TGF-βPromotes the Growth of Bovine Chondrocytes in Monolayer Culture and the Formation of Cartilage Tissue on Three-Dimensional ScaffoldsTissue Engineering, 1995
- Responsiveness of articular cartilage from normal and inflamed mouse knee joints to various growth factors.Annals Of The Rheumatic Diseases, 1994
- Increased damage to type II collagen in osteoarthritic articular cartilage detected by a new immunoassay.JCI Insight, 1994
- Peptide growth factors and their interactions during chondrogenesisProgress in Growth Factor Research, 1992
- Effect of transforming growth factor-β1 (TGF-β1) on matrix synthesis by monolayer cultures of rabbit articular chondrocytes during the dedifferentiation processExperimental Cell Research, 1992
- Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gelsCell, 1982