Synthesis of Cholesteryl Supports and Phosphoramidite for Automated DNA Synthesis of Triple-Helix Forming Oligonucleotides (Tfos)
- 1 September 1993
- journal article
- research article
- Published by Taylor & Francis in Nucleosides and Nucleotides
- Vol. 12 (8), 853-864
- https://doi.org/10.1080/07328319308018556
Abstract
DMT-Cholesteryl succinylamino solid supports (CPG, loading: 33 μmole/gram and TentaGel loading: 152 μmole/gram) and DMT-cholesteryl phosphoramidite were prepared for use in automated DNA synthesis of cholesteryl modified TFOs in the synthesis scales from 0.2 to 300 μmole. The modified TFOs were found to have a 5 to 50 fold increase in their uptake properties.Keywords
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