Interleukin 1 production by human polymorphonuclear neutrophils.
- 15 May 1986
- journal article
- research article
- Published by The American Association of Immunologists in The Journal of Immunology
- Vol. 136 (10), 3677-3685
- https://doi.org/10.4049/jimmunol.136.10.3677
Abstract
The purpose of this study was to determine whether human polymorphonuclear neutrophils (PMN), which share a common cell lineage with macrophages, could produce factors such as IL 1. Other properties which these two cell types share are their phagocytic nature and the common receptor and antigens on their cell surfaces. IL 1, in many of its physical, biochemical, and functional characteristics, is found to resemble endogenous pyrogen (EP). PMN have been cited as a possible cell source of EP, but there have also been reports in which the capacity of PMN to produce EP has been questioned. This study shows that normal human PMN can be stimulated by particulate agents such as zymosan and soluble agents such as phorbol myristic acetate to produce a factor(s) which induces proliferation of mouse thymocytes, i.e., PMN IL 1. This PMN IL 1 was released from PMN in a dose- and time-dependent fashion. PMN IL 1 was nondialyzable, was heat-labile, and was inactivated at pH below 5 and above 8. PMN IL 1 stimulated the proliferation of normal human synovial fibroblasts and caused release of a neutral protease (plasminogen activator) from synovial cells. The synovial and thymocyte-proliferating capacity of PMN IL 1 was not affected by the protease inhibitor aprotinin or by soybean trypsin inhibitor. Gel filtration studies estimate the m.w. of PMN IL 1 to be approximately 13,000 to 17,000.This publication has 20 references indexed in Scilit:
- Ability of human leukocytic pyrogen to enhance phytohemagglutinin induced murine thymocyte proliferationCellular Immunology, 1981
- Stimulation of rheumatoid synovial cell collagenase and prostaglandin production by partially purified lymphocyte-activating factor (interleukin 1).Proceedings of the National Academy of Sciences, 1981
- Failure of rabbit neutrophils to secrete endogenous pyrogen when stimulated with staphylococci.The Journal of Experimental Medicine, 1980
- Connective tissue activationArthritis & Rheumatism, 1980
- A PMN-derived factor that enhances DNA-synthesis in PHA or antigen-stimulated lymphocytes.The Journal of Immunology, 1979
- Secretion of plasminogen activator by human polymorphonuclear leukocytes. Modulation by glucocorticoids and other effectors.The Journal of Experimental Medicine, 1977
- In vitro stimulation of lymphocytes by neutral proteinases from human polymorphonuclear leukocyte granules.The Journal of Experimental Medicine, 1976
- INTERACTION BETWEEN LYMPHOCYTES AND INFLAMMATORY EXUDATE CELLS .2. PROTEOLYTIC-ENZYME RELEASED BY PMN AS A POSSIBLE MEDIATOR FOR ENHANCEMENT OF THYMOCYTE RESPONSE1976
- STUDIES ON THE PATHOGENESIS OF FEVERThe Journal of Experimental Medicine, 1953
- The polymerization of proteins: the action of thrombin on fibrinogenArchives of Biochemistry and Biophysics, 1951