Isolation and Some Properties of NAD+ Reductase of the Green Photosynthetic Bacterium Prosthecochloris aestuarii
- 1 February 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 79 (2), 361-371
- https://doi.org/10.1093/oxfordjournals.jbchem.a131079
Abstract
NAD+ reductase of the green photosynthetic bacterium Prosthecochloris aestuarii was isolated and purified by ammonium sulfate fractionation, DEAE-cellulose column chromatography, and Sephadex G-200 gel filtration. This enzyme is an FAD-containing flavoprotein and has absorption maxima at 485 (shoulder), 452, 411, and 385 nm (the 411 nm band is due to cytochrome). The molecular weight of the enzyme as determined by gel filtration using Sephadex G-200 is 119, 000. The enzyme catalyzes the reduction of NAD+ and NADP+ by photoreduced spinach ferredoxin or reduced benzyl viologen. It also catalyzes the reduction of cytochromes and dyes such as benzyl viologen and 2, 6-dichloroindophenol (DCIP) with NADH or NADPH as the electron donor. The reduction of cytochrome c-555(550) of this organism is accelerated by the addition of cofactors such as menadione. In these reactions, the enzyme is more specific for NAD+ or NADH than for NADP+ or NADPH. Reduction of cytochrome c or dyes catalyzed by the enzyme is strongly inhibited by rotenone or amytal, but not by antimycin A or o-phenanthroline. It is suggested that NAD+ photoreduction in this organism takes place via direct electron flow with the mediation of ferredoxin and flavoprotein enzyme, as in Chloro-bium and green plants.This publication has 19 references indexed in Scilit:
- [39] Ferredoxins from photosynthetic bacteria, algae, and higher plantsPublished by Elsevier ,1971
- Enzymic Mechanisms of Pyridine Nucleotide Reduction in ChloroplastsJournal of Biological Chemistry, 1965
- Estimation of the molecular weights of proteins by Sephadex gel-filtrationBiochemical Journal, 1964
- CRYSTALLIZATION OF FERREDOXIN-TPN REDUCTASE AND ITS ROLE IN PHOTOSYNTHETIC APPARATUS OF CHLOROPLASTS1963
- Über die Atmungsfermente derChlorellaHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1962
- A New Hematin Compound Isolated from Euglena GracilisThe Journal of Biochemistry, 1959
- TPNH-cytochrome c reductase of chloroplasts and its role in photosynthetic phosphorylationArchives of Biochemistry and Biophysics, 1958
- A SPECIFIC SPECTROPHOTOMETRIC ASSAY FOR FLAVIN ADENINE DINUCLEOTIDEJournal of Biological Chemistry, 1956
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951
- THE EXTINCTION COEFFICIENTS OF THE REDUCED BAND OF PYRIDINE NUCLEOTIDESJournal of Biological Chemistry, 1948