Chemical factors that influence nucleocytoplasmic transport: a fluorescence photobleaching study.
Open Access
- 1 March 1986
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 102 (3), 853-858
- https://doi.org/10.1083/jcb.102.3.853
Abstract
The technique of fluorescence redistribution after photobleaching was used to measure the translocation rate of fluorescein-labeled dextrans across the nuclear pore complex in isolated rat liver nuclei. A transport assay system was established that could monitor the effect of biologically active molecules, e.g., ATP, GTP, cAMP on the translocation process. The results show that ATP, phosphoinositides, RNA, and insulin can enhance transport rates from 195 to 432%. It was further demonstrated that concanavalin A, but not wheat germ or soybean agglutinin, can block dextran transport completely. The effectors of dextran transport are similar to substances demonstrated to effect the efflux of RNA from isolated nuclei. A model for translocation through the nuclear pore is now presented that incorporates data from protein influx and RNA efflux experiments into a single pathway controlled by ATP.This publication has 35 references indexed in Scilit:
- Insulin Stimulation of Nucleoside Triphosphatase Activity in Isolated Nuclear EnvelopesScience, 1982
- A large particle associated with the perimeter of the nuclear pore complex.The Journal of cell biology, 1982
- Insulin-modulated transport of RNA from isolated liver nucleiArchives of Biochemistry and Biophysics, 1981
- Pertinence of nuclear envelope nucleoside triphosphatase activity to ribonucleic acid transportBiochemistry, 1980
- Influence of nucleotides, cations and nucleoside triphosphatase inhibitors on the release of ribonucleic acid from isolated rat liver nucleiBiochemical Journal, 1980
- Identification of Concanavalin A binding glycoproteins of rat liver cell nuclear membranesBiochemical and Biophysical Research Communications, 1977
- The nuclear and the cytoplasmic pore complex: structure, dynamics, distribution, and evolution.1977
- Nuclear envelope permeabilityNature, 1975
- STRUCTURE OF MACRONUCLEAR ENVELOPES OF TETRAHYMENA PYRIFORMIS IN THE STATIONARY PHASE OF GROWTHThe Journal of cell biology, 1968
- RNA TRANSPORT FROM NUCLEUS TO CYTOPLASM IN CHIRONOMUS SALIVARY GLANDSThe Journal of cell biology, 1966