Replication of Rubella Virus in a Continuous Line of African Green Monkey Kidney Cells (Vero).

Abstract

Rubella virus grew to high titer in a continuous line of green monkey kidney cells (Vero), with the production of CPE [cytopathogenic effect] and plaques. Infectivity titrations can be read within 2 weeks and are somewhat higher than comparable titers measured by echovirus 11 interference in pGMK [primary green monkey kidney]. Primary isolation rates of 56% were obtained in Vero cultures as compared to 12% with the same specimens tested in pGMK cultures. Complement fixing antigen can be produced in satisfactory titer in Vero cultures, and the system is suitable for the direct measurement of neutralizing antibody.