Serine Transhydroxymethylase from Rabbit Liver
- 1 November 1976
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 70 (2), 397-401
- https://doi.org/10.1111/j.1432-1033.1976.tb11029.x
Abstract
The amino acid sequence of the coenzyme-binding site of serine transhydroxymethylase from rabbit liver was determined. After reduction with NaBH4 and aminoethylation, a 1st sample of enzyme was digested with thermolysin [EC 3.4.2.4.4] and a single phosphopyridoxyl peptide was isolated. A 2nd sample of similarly treated enzyme was digested with chymotrypsin [EC 3.4.21.1] and 3 phosphopyridoxyl peptides clearly originating from a unique coenzyme-binding site were isolated. Sequence analysis of these peptides indicate the following structure: Val-Val-Thr-Thr-Thr-His-Lys(Pxy)-Thr-Leu. Sequence homologies of the active site of various pyridoxalphosphate enzymes are discussed in terms of a possible catalytic role and of evolution of this class of proteins.This publication has 31 references indexed in Scilit:
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