The requirement for membrane sialic acid in the stimulation of superoxide production during phagocytosis by human polymorphonuclear leukocytes.
Open Access
- 1 June 1976
- journal article
- research article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 143 (6), 1308-1316
- https://doi.org/10.1084/jem.143.6.1308
Abstract
The effect of desialylation on phagocytosis of latex particles and oxidative metabolism of human polymorphonuclear leukocytes was studied. Removal of 20% total leukocyte sialic acid by bacterial neuraminidase had no effect on phagocytosis of latex particles and phagocytosis-associated activation of hexose monophosphate shunt in human polymorphonuclear leukocytes. In contrast, desialylation prevented the stimulation of superoxide production either by phagocytosis or by concanavalin A. It is concluded that membrane sialic acid is essential for the stimulation of superoxide production by human polymorphonuclear leukocytes.This publication has 23 references indexed in Scilit:
- Complement and immunoglobulins stimulate superoxide production by human leukocytes independently of phagocytosis.Journal of Clinical Investigation, 1975
- Pyridine nucleotide-dependent superoxide production by a cell-free system from human granulocytes.Journal of Clinical Investigation, 1975
- Defect in Pyridine Nucleotide Dependent Superoxide Production by a Particulate Fraction from the Granulocytes of Patients with Chronic Granulomatous DiseaseNew England Journal of Medicine, 1975
- The role of superoxide anion and hydrogen peroxide in phagocytosis-associated oxidative metabolic reactions.Journal of Clinical Investigation, 1975
- Antimicrobial mechanisms in neutrophilic polymorphonuclear leukocytes.1975
- NADPH oxidase deficiency in X-linked chronic granulomatous disease.Journal of Clinical Investigation, 1975
- An isotopic assay for NADPH oxidase activity and some characteristics of the enzyme from human polymorphonuclear leukocytes.Journal of Clinical Investigation, 1975
- Reversible metabolic stimulation of polymorphonuclear leukocytes and macrophages by concanavalin A.1973
- The Thiobarbituric Acid Assay of Sialic AcidsJournal of Biological Chemistry, 1959
- BIOCHEMICAL BASIS OF PHAGOCYTOSIS .1. METABOLIC CHANGES DURING THE INGESTION OF PARTICLES BY POLYMORPHONUCLEAR LEUKOCYTES1959