A COLORIMETRIC METHOD FOR THE DETERMINATION OF BUTYRIC ACID

Abstract
A colorimetric method for estimation of butyric acid in presence of acetic and formic acids is described. The solution to be tested is made to a definite strength, and is then treated with a CuCl2-HCl reagent and the mixture shaken with chloroform. Known mixtures of butyric and acetic acids are treated in the same manner and are used as standards for comparison. By carefully standardizing the procedure good checks were obtained with the Duclaux method on 17 cultures producing butyric acid.