Laboratory and Field Methods for Assaying Olfactory Responses of the Douglas-fir Beetle, Dendroctonus pseudotsugae Hopkins

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Abstract
Laboratory tests revealed that female Douglas-fir beetles, Dendroctonus pseudotsugae Hopkins, boring in logs of both host and non-host species produce a volatile substance that both attracts and arrests adult beetles. Male arrestment provided a reliable method for laboratory bioassay of volatile substances. Field tests were devised to show the attack, the attraction produced, and the broad development in six tree species: Douglas-fir, Pseudotsuga menziesii (Mirb.) Franco; western larch, Larix occidentalis Nutt.; ponderosa pine, Pinus ponderosa Laws.; western hemlock, Tsuga heteropbylla (Raf.) Sarg.; western white pine, Pinus monticola Dougl.; and grand fir, Abies grandis (Dougl.) Lindl.