Endoglycanase-Catalyzed Degradation of Hemicelluloses during Development of Carnation (Dianthus caryophyllus L.) Petals

Abstract

Large molecular-size hemicelluloses, including xyloglucan, decreased in quantity during development of carnation (Dianthus caryophyllus L. cv White Sim) petals, along with a relative increase in polymers with an average size of 10 kilodaltons. An enzyme extract from senescing petal tissue depolymerized the large molecular-size hemicelluloses in a pattern similar to that occurring in vivo during petal development. The products generated in vitro were composed of polymeric and monomeric components, the latter consisting primarily of xylose, galactose, and glucose. The 10 kilodalton hemicelluloses were resistant to in vitro enzymic hydrolysis. Glycosyl-linkage composition of the large molecular-size polymers provided evidence for the presence of xyloglucan with smaller amounts of arabinoxylan and arabinan. The 10 kilodalton polymers were enriched in mannosyl and 4-linked glucosyl residues, presumably derived from glucomannan. During petal development or enzymic hydrolysis, no change was observed in the relative glycosyl-linkage composition of the large molecular-size hemicelluloses. The in vitro activity of carnation petal enzymes active toward native hemicelluloses increased threefold at the onset of senescence and declined slightly thereafter. Gel chromatography revealed 23 and 12 kilodalton proteins with hemicellulase activity. The enzymes hydrolyzed the large molecular-size hemicelluloses extensively and without formation of monomers. Endoxylanase activity was detected in the partially purified enzyme preparation. Xyloglucan was depolymerized in the absence of cellulase activity, suggesting the presence of a xyloglucan-specific glucanase. These data indicate that the hemicellulose molecular-size changes observed during development of carnation petals are due, in part, to the enzymic depolymerization of large molecular-size hemicelluloses.