Expression and processing of bacterial beta-lactamase in the yeast Saccharomyces cerevisiae.
- 1 July 1981
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 78 (7), 4466-4470
- https://doi.org/10.1073/pnas.78.7.4466
Abstract
The mode of expression in S. cerevisiae of the bacterial antibiotic resistance gene coding for .beta.-lactamase (EC 3.5.2.6) is described. Yeast transformants, containing hybrid plasmid pMP78-1 consisting of the Escherichia coli plasmid pBR325 in a 2-.mu.m DNA vector, synthesize an active .beta.-lactamase protein. The enzyme was purified .apprx. 100-fold over crude extracts. With regard to activity, MW and binding to specific antibodies, the yeast .beta.-lactamase was indistinguishable from the purified enzyme from E. coli. Because the bacterial enzyme is synthesized as a preprotein with subsequent maturation, S. cerevisiae may convert the preprotein to the mature .beta.-lactamase. This was confirmed by in vitro experiments showing that the bacterial preprotein can be processed by crude extracts of S. cerevisiae.This publication has 18 references indexed in Scilit:
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