The vitamin B12-binding systems of isolated intestine of the rat
- 1 April 1958
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 68 (4), 592-597
- https://doi.org/10.1042/bj0680592
Abstract
The measurement of uptake of radioactive vitamin B12 by isolated intestine has been described. The effect of pseudovitamin B12, an anti-vitamin B12 and the lactone of vitamin B12 on uptake of radioactive vitamin B12 has been studied. Two vitamin B12-binding systems were demonstrated, one competitive and the other non-competitive. The implications of the non-competitive binding system in relation vitamin B12 uptake are discussed.Keywords
This publication has 4 references indexed in Scilit:
- The activity for chicks of some vitamin B12-like compoundsBiochemical Journal, 1956
- Intrinsic factor studies. IV. Selective absorption and binding of cyanocobalamin by gastric juice in the presence of excess pseudovitamin B12 or 5, 6-dimethylbenzimidazole.1956
- Comparative Absorption of Vitamin B12 Analogues by Normal Humans. II. Chloro-, Sulfato-, Nitro- and Thiocyanato- vs CyanocobalaminExperimental Biology and Medicine, 1956
- The use of sacs of everted small intestine for the study of the transference of substances from the mucosal to the serosal surfaceThe Journal of Physiology, 1954