Dissociation, Reassociation, and Purification of Plastid and Cytosolic Phosphoglucose Isomerase Isozymes

Abstract

The plastid and cytosolic isozymes of the dimeric enzyme phosphoglucose isomerase (EC 5.3.1.9) from spinach (Spinacia oleracea) and cauliflower (Brassica oleracea) were purified to apparent homogeneity. The isozymes from sunflower (Helianthus annuus) and Clarkia xantiana were partially purified. When subunits from 2 electrophoretically distinguishable cytosolic isozymes, either from the same or from different species, were dissociated and allowed to reassociate in each other''s presence, an active hybrid enzyme, consisting of 1 subunit of each type, was formed in addition to the 2 original homodimers. Active hybrid enzymes were formed by dissociation and reassociation of plastid isozymes. Hybrid molecules were not produced between the plastid and cytosolic subunits, suggesting that they are not able to bind with each other. Additional differences between the plastid and cytosolic isozymes are described.