The Isolation of Chondroitin Sulfuric Acid from Dentin
- 1 December 1952
- journal article
- research article
- Published by SAGE Publications in Journal of Dental Research
- Vol. 31 (6), 793-797
- https://doi.org/10.1177/00220345520310061001
Abstract
Chondroitin sulfuric acid was isolated as its K salt from normal human dentin by the method applied to cartilage by Einbinder and Schubert (Jour. Biol. Chem. 185: 624. 1950) with a yield of 0.64%. Hexosamine and hexuronic acid detns. upon intact dentin gave a value of approx. 0.3%. However, the analytical procedures for these compounds give low results due to destruction by acid hydrolysis in the presence of large amts. of inorganic salts. The presence of galactosamine in the chondroitin sulfuric acid was shown by means of paper chrc-matography, indicating its similarity to chondroitin isolated from cartilage.Keywords
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