Affinity Chromatographic Quantitation of Glycosylated Hemoglobin in Newborn Infants

Abstract

Levels of glycosylated hemoglobin (glyco Hb) were determined in 45 newborn infants by affinity chromatography on phenylboro-nate-agarose gel and found to correlate well with the results obtained by two chemical methods. However the percentages of Hb F_Iad˚ and Hb F_Ic determined by Bio-Rex 70 chromatography did not correlate with the glyco Hb values. The glyco Hb levels in the newborns were significantly lower than in the adults. Rechromatography of the glyco and nonglyco Hb fractions on Bio-Rex 70 columns indicated that Hb F -like glyco Hb (presumably formed by glycosylation of the α chain amino terminus and e-amino groups of lysyl amino acids) constituted about 60% of the glyco Hb fraction, whereas both Hb F_Ic and Hb F_Oad˚ components contained only small portions of the glyco Hb.