Topostin, a novel inhibitor of mammalian DNA topoisomerase I from Flexibacter topostinus sp. nov. II. Purification and some properties of topostin.
- 1 January 1990
- journal article
- research article
- Published by Japan Antibiotics Research Association in The Journal of Antibiotics
- Vol. 43 (2), 158-162
- https://doi.org/10.7164/antibiotics.43.158
Abstract
We describe the isolation of inhibitors of mammalian DNA topoisomerase I, named topostins, from a culture broth of Flexibacter topostinus sp. nov. and some properties of the inhibitors. Topostins Al, A2 and B were isolated by differential solubility in solvents, adsorption chromatography on silica gel and gel nitration on a Sephadex LH-20 column. Topostins Al, A2 and B had specific activities of 4, 700, 16, 000 and 22, 000 U/mg, respectively. The most active metabolite topostin B comprised two components with MW of 567 and 553 in an equimolar ratio.This publication has 2 references indexed in Scilit:
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- DNA Gyrase and the Supercoiling of DNAScience, 1980